Hep_Ploidy_protocol

This is the ploidy identification workflow from the article "Stereo-cell Deciphers the Spatial and Functional Heterogeneity of Polyploid Hepatocytes". The method employs deep learning techniques: Cellpose & StarDist to accurately identify DAPI fluorescence-stained images and brightfield images, respectively. It acquires detailed information on the morphological characteristics and spatial localization of nuclei and cells, which serves as the core process for hepatocyte ...