MPXV (Mpox) Phylogenetic Analysis with Squirrel

Description

Galaxy workflow to perform MPXV phylogenetic reconstruction using the Squirrel (Some QUIck Reconstruction to Resolve Evolutionary Links) Galaxy tools, Squirrel QC and Squirrel Phylo. The workflow also performs masking (using the squirrel tool) of SNPs.

Galaxy Squirrel tools:

• Squirrel QC: The quality control (QC) mode of Squirrel that can run QC on the alignment and flag certain sites ...

Viral Amplicon Analysis Pipeline for ONT Data

Description

Galaxy workflow for processing viral amplicon datasets, such as MPXV (Mpox), sequenced using the Oxford Nanopore Technologies (ONT) platform. The workflow uses the latest Fieldbioinformatics Artic Minion pipeline that uses the clair3 variant caller.

The ARTIC minion is a pipeline for working with viral nanopore sequencing data, generated from tiling amplicon schemes. It is designed ...

Using:

• vadr annotation (model to select)
• vardict variant caller
• coverage depth

Provides summarizing files:

• png image of variant calling with annotations and coverage depths
• tsv file with all information of significant variants only
• vcf file with all information of significant variants only (to allow downstream NextStrain analyses)

Using:

• vadr annotation (model to select)
• vardict variant caller
• coverage depth

Provides summarizing files:

• png image of variant calling with annotations and coverage depths
• tsv file with all information of significant variants only
• vcf file with all information of significant variants only (to allow downstream NextStrain analyses)

Using:

• vadr annotation (model to select)
• vardict variant caller
• coverage depth

Provides summarizing files:

• png image of variant calling with annotations and coverage depths
• tsv file with all information of significant variants only
• vcf file with all information of significant variants only (to allow downstream NextStrain analyses)

Using:

• vadr annotation (model to select)
• vardict variant caller
• coverage depth

Provides summarizing files:

• png image of variant calling with annotations and coverage depths
• tsv file with all information of significant variants only
• vcf file with all information of significant variants only (to allow downstream NextStrain analyses)

Assembly and intrahost/low-frequency variant calling for viral samples

Integration of viral sequences in genomic data

ONTViSc (ONT-based Viral Screening for Biosecurity)

Introduction

eresearchqut/ontvisc is a Nextflow-based bioinformatics pipeline designed to help diagnostics of viruses and viroid pathogens for biosecurity. It takes fastq files generated from either amplicon or whole-genome sequencing using Oxford Nanopore Technologies as input.

The pipeline can either: 1) perform a direct search on the sequenced reads, 2) generate clusters, 3) assemble the reads to generate longer contigs or 4) directly ...

PAIRED-END workflow. Align reads on fasta reference/assembly using bwa mem, get a consensus, variants, mutation explanations.

IMPORTANT:

• For "bcftools call" consensus step, the --ploidy file is in "Données partagées" (Shared Data) and must be imported in your history to use the worflow by providing this file (tells bcftools to consider haploid variant calling).
• SELECT THE MOST ADAPTED VADR MODEL for annotation (see vadr parameters).

SINGLE-END workflow. Align reads on fasta reference/assembly using bwa mem, get a consensus, variants, mutation explanations.

IMPORTANT:

• For "bcftools call" consensus step, the --ploidy file is in "Données partagées" (Shared Data) and must be imported in your history to use the worflow by providing this file (tells bcftools to consider haploid variant calling).
• SELECT the mot ADAPTED VADR MODEL for annotation (see vadr parameters).

Non-functional workflow to get a global view of possibilities for plant virus classification.