Using:
- vadr annotation (virus model must be selected in options)
- vardict variant caller
- coverage depth Provides summarizing files:
- png image of variant calling with annotations and coverage depths
- tsv file with all information of significant variants only
Type: Galaxy
Creators: Fabrice Touzain, This study was founded by the French National Research Agency and by Santé publique France as part of the project "EMERGEN". Anses Ploufragan research was also supported by Agglomération de Saint-Brieuc, Département des Côtes d'Armor and Région Bretagne
Submitter: Fabrice Touzain
Using:
- vadr annotation (virus model must be selected in options)
- vardict variant caller
- coverage depth Provides summarizing files:
- png image of variant calling with annotations and coverage depths
- tsv file with all information of significant variants only
Type: Galaxy
Creators: Fabrice Touzain, This study was founded by the French National Research Agency and by Santé publique France as part of the project "EMERGEN". Anses Ploufragan research was also supported by Agglomération de Saint-Brieuc, Département des Côtes d'Armor and Région Bretagne
Submitter: Fabrice Touzain
Using:
- vadr annotation (virus model must be selected in options)
- vardict variant caller
- coverage depth Provides summarizing files:
- png image of variant calling with annotations and coverage depths
- tsv file with all information of significant variants only (can be opened in Excel/LibreOffice)
Type: Galaxy
Creators: Fabrice Touzain, This study was founded by the French National Research Agency and by Santé publique France as part of the project "EMERGEN". Anses Ploufragan research was also supported by Agglomération de Saint-Brieuc, Département des Côtes d'Armor and Région Bretagne
Submitter: Fabrice Touzain
Using:
- vadr annotation (virus model must be selected in options)
- vardict variant caller
- coverage depth Provides summarizing files:
- png image of variant calling with annotations and coverage depths
- tsv file with all information of significant variants only (can be opened in Excel/LibreOffice)
Type: Galaxy
Creators: Fabrice Touzain, This study was founded by the French National Research Agency and by Santé publique France as part of the project "EMERGEN". Anses Ploufragan research was also supported by Agglomération de Saint-Brieuc, Département des Côtes d'Armor and Région Bretagne
Submitter: Fabrice Touzain
Assembly and intrahost/low-frequency variant calling for viral samples
Type: Nextflow
Creators: Sarai Varona and Sara Monzon, Patel H, Varona S and Monzon S
Submitter: WorkflowHub Bot
Integration of viral sequences in genomic data
ONTViSc (ONT-based Viral Screening for Biosecurity)
Introduction
eresearchqut/ontvisc is a Nextflow-based bioinformatics pipeline designed to help diagnostics of viruses and viroid pathogens for biosecurity. It takes fastq files generated from either amplicon or whole-genome sequencing using Oxford Nanopore Technologies as input.
The pipeline can either: 1) perform a direct search on the sequenced reads, 2) generate clusters, 3) assemble the reads to generate longer contigs or 4) directly ...
Type: Nextflow
Creators: Marie-Emilie Gauthier, Craig Windell, Magdalena Antczak, Roberto Barrero
Submitter: Magdalena Antczak
PAIRED-END workflow. Align reads on fasta reference/assembly using bwa mem, get a consensus, variants, mutation explanations.
IMPORTANT:
- For "bcftools call" consensus step, the --ploidy file is in "Données partagées" (Shared Data) and must be imported in your history to use the worflow by providing this file (tells bcftools to consider haploid variant calling).
- SELECT THE MOST ADAPTED VADR MODEL for annotation (see vadr parameters).
SINGLE-END workflow. Align reads on fasta reference/assembly using bwa mem, get a consensus, variants, mutation explanations.
IMPORTANT:
- For "bcftools call" consensus step, the --ploidy file is in "Données partagées" (Shared Data) and must be imported in your history to use the worflow by providing this file (tells bcftools to consider haploid variant calling).
- SELECT the mot ADAPTED VADR MODEL for annotation (see vadr parameters).
Non-functional workflow to get a global view of possibilities for plant virus classification.