Workflow Type: Galaxy

encyclopedia- DIA Metaproteomics

Associated Tutorial

This workflows is part of the tutorial EncyclopeDIA, available in the GTN

Features

Thanks to...

Workflow Author(s): GalaxyP

Tutorial Author(s): Emma Leith, Subina Mehta, James Johnson, Pratik Jagtap, Timothy J. Griffin

gtn star logo followed by the word workflows

Inputs

ID Name Description Type
BACKGROUND PROTEIN FASTA FILE #main/BACKGROUND PROTEIN FASTA FILE Input Protein Database file
  • File
EXPERIMENTAL DIA RAW FILES (WIDE) #main/EXPERIMENTAL DIA RAW FILES (WIDE) Input raw files
  • array containing
    • File
GPF DIA RAW Files (Dataset collection) #main/GPF DIA RAW Files (Dataset collection) encyclopedia- Gas phase fractionation files for library
  • array containing
    • File
SPECTRAL OR PROSIT LIBRARY #main/SPECTRAL OR PROSIT LIBRARY Library from Prosit
  • File

Steps

ID Name Description
4 Converting RAW to mzml convert to mzml for encyclopedia toolshed.g2.bx.psu.edu/repos/galaxyp/msconvert/msconvert/3.0.20287.2
5 msconvert toolshed.g2.bx.psu.edu/repos/galaxyp/msconvert/msconvert/3.0.20287.2
6 Library searching Searching the mzml files against the Prosit library and the protein FASTA database. toolshed.g2.bx.psu.edu/repos/galaxyp/encyclopedia_searchtolib/encyclopedia_searchtolib/1.2.2+galaxy0
7 EncyclopeDIA Quantify toolshed.g2.bx.psu.edu/repos/galaxyp/encyclopedia_quantify/encyclopedia_quantify/1.2.2+galaxy0

Outputs

ID Name Description Type
CHROMATOGRAM LIBRARY #main/CHROMATOGRAM LIBRARY n/a
  • File
Experimental-dataset-MZML #main/Experimental-dataset-MZML n/a
  • File
GPF_MZML #main/GPF_MZML n/a
  • File
LOG #main/LOG n/a
  • File
PEPTIDE QUANTITATION OUTPUT #main/PEPTIDE QUANTITATION OUTPUT n/a
  • File
PROTEIN QUANTITATION OUTPUT #main/PROTEIN QUANTITATION OUTPUT n/a
  • File
QUANT LIBRARY #main/QUANT LIBRARY n/a
  • File
Quant-LOG #main/Quant-LOG n/a
  • File

Version History

2.0 (latest) Created 9th Sep 2024 at 14:23 by Helena Rasche

Added/updated 4 files


Open master e1af47d

1.0 Created 16th Jul 2024 at 14:07 by Helena Rasche

Added/updated 4 files


Frozen 1.0 a09fe37

4.0 (earliest) Created 25th Jun 2024 at 11:03 by Helena Rasche

Added/updated 4 files


Frozen 4.0 144d8a5
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Activity

Views: 240   Downloads: 101

Created: 25th Jun 2024 at 11:03

Last updated: 9th Sep 2024 at 14:23

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Total size: 107 KB
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