Workflow Type: Common Workflow Language
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Transcripts annotation workflow
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Inputs
ID | Name | Description | Type |
---|---|---|---|
transcriptsFile | n/a | n/a |
|
singleBestOnly | n/a | n/a |
|
replace | n/a | n/a |
|
diamondSeqdb | n/a | n/a |
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i5_chunk_size | n/a | n/a |
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i5Databases | n/a | n/a |
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i5Applications | n/a | n/a |
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i5OutputFormat | n/a | n/a |
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blockSize | n/a | n/a |
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covariance_models | n/a | n/a |
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clanInfoFile | n/a | n/a |
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cmsearchCores | n/a | n/a |
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buscoMode | n/a | n/a |
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buscoOutputName | n/a | n/a |
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buscoLineage | n/a | n/a |
|
Steps
ID | Name | Description |
---|---|---|
cut_fasta_header | Cuts FASTA headers which are too long | n/a |
clean_fasta_header | Replaces problematic characters from FASTA headers with dashes | n/a |
identify_coding_regions | Identifies candidate coding regions within transcript sequences | n/a |
remove_asterisks_and_reformat | Removes asterisks characters from given peptide sequences | n/a |
functional_analysis | n/a | Matches are generated against predicted CDS, using a sub set of databases from InterPro. |
calculate_diamond_matches | Calculates Diamond matches | n/a |
identify_nc_rna | Identifies non-coding RNAs using Rfams covariance models | n/a |
run_transcriptome_assessment | Performs transcriptome assessment using BUSCO | n/a |
Outputs
ID | Name | Description | Type |
---|---|---|---|
cutted_transcripts_file | n/a | n/a |
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cleaned_transcripts_file | n/a | n/a |
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peptide_sequences | n/a | n/a |
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coding_regions | n/a | n/a |
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gff3_output | n/a | n/a |
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bed_output | n/a | n/a |
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reformatted_sequences | n/a | n/a |
|
i5Annotations | n/a | n/a |
|
diamond_matches | n/a | n/a |
|
deoverlapped_matches | n/a | n/a |
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busco_short_summary | n/a | n/a |
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busco_full_table | n/a | n/a |
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busco_missing_buscos | n/a | n/a |
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busco_hmmer_output | n/a | n/a |
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busco_translated_proteins | n/a | n/a |
|
busco_blast_output | n/a | n/a |
|
Version History
Version 1 (earliest) Created 25th Feb 2020 at 13:18 by Finn Bacall
Added/updated 26 files
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master
41d0b78
Creators and Submitter
Creators
Not specifiedAdditional credit
Arnaud Meng, Maxim Scheremetjew, Michael Crusoe
Submitter
License
Activity
Views: 1699 Downloads: 309
Created: 25th Feb 2020 at 13:18
Last updated: 25th Feb 2020 at 15:17
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