Welcome to the iBOL Europe Space!

Here we collect, curate and develop SOPs and pipelines to extract, sequence, assemble and publish DNA barcodes, genome skims and DNA metabarcodes for all eukaryotic life.

For fresh sample barcoding head to our Barcoding Team

For museum specimen genome skimming head to our Museum Skimming Team

For barcode data wrangling head to our Barcode Data Team ...

This workflow uses a slightly modified version of the Santa Cruz Reaction (SCR) protocol by Kapp et al. 2021 for library building of museum specimens. The reaction volumes can be halved to reduce reagent costs, lower volumes have not been tested yet. Please cite the original publication if using this method: https://doi.org/10.1093/jhered/esab012

NGS Equimolar Pooling Calculator

A web-based tool for calculating equimolar pooling volumes from complex sub-pools for Next-Generation Sequencing (NGS).

About

This calculator helps researchers determine the correct volumes to take from each pool when combining multiple complex sub-pools of NGS libraries for sequencing. It ensures that each individual sample contributes an equal number of molecules to the final sequencing run, resulting in more balanced coverage.

Features

• Calculate ...

Gene_fetch

This tool fetches gene sequences from NCBI databases based on taxonomy IDs (taxids) or taxonomic information. It can retrieve both protein and nucleotide sequences for various genes, including protein-coding genes (e.g., cox1, cytb, rbcl, matk) and rRNA genes (e.g., 16S, 18S).

Feature highlight

• Fetch protein and/or nucleotide sequences from NCBI GenBank database.
• Handles both direct nucleotide sequences and protein-linked nucleotide searches (CDS extraction includes fallback ...

Gene_fetch

This tool fetches gene sequences from NCBI databases based on taxonomy IDs (taxids) or taxonomic information. It can retrieve both protein and nucleotide sequences for various genes, including protein-coding genes (e.g., cox1, cytb, rbcl, matk) and rRNA genes (e.g., 16S, 18S).

Feature highlight

• Fetch protein and/or nucleotide sequences from NCBI GenBank database.
• Handles both direct nucleotide sequences and protein-linked nucleotide searches (CDS extraction includes fallback ...