vvv2_align_SE, vvv2_align_PE/vvv2_display: Galaxy-Based Workflows and Tool Designed to Perform, Summarize and Visualize Variant Calling and Annotation in Viral Genome Assemblies
View Publication
Export 
BiBTeXBackground: Next-generation sequencing (NGS) analysis of viral samples generates results dispersed across multiple files—genome assembly, variant calling, and functional annotations—making integrated interpretation challenging. Variants often yield numerous low-frequency or non-significant variants, yet only a small fraction are biologically relevant. Virologists must manually sift through extensive data to identify meaningful mutations, a time-consuming and error-prone process. To address these practical challenges, we developed vvv2_display, a dedicated summarization and visualization tool, integrated within comprehensive Galaxy workflows. Results: vvv2_display streamlines variant interpretation by consolidating key results into two concise and interoperable outputs. The first output is a PNG image showing alignment coverage depth and genomic annotations, with significant variants displayed along the genome as symbols whose height reflects frequency and shape indicates the affected protein. At a glance, this enables virologists to identify all deviations from a reference viral genome. Each significant variant is assigned a unique identifier that directly links to the second output: a tab-separated (TSV) text file listing only high-confidence variants, with frequencies, flanking nucleotides, and impacted genes and proteins. This cross-referenced design supports rapid, accurate, and intuitive data exploration. Availability: vvv2_display is open source, available on Github and installable via Mamba.
SEEK ID: https://workflowhub.eu/publications/66
DOI: 10.3390/v17101385
Teams: ANSES-Ploufragan
Publication type: Journal Article
Journal: Viruses
Book Title: Viruses
Publisher: MDPI AG
Citation: Viruses 17(10):1385.
Date Published: 17th Oct 2025
Registered Mode: by DOI
Export PNG
Download
Submitter
Views: 271
Created: 1st Apr 2026 at 17:00
TagsThis item has not yet been tagged.
AttributionsNone
Related items
https://orcid.org/0000-0002-1103-2425
A space managed by WorkflowHub administrators for teams that don't want/need to manage their own space.
Teams: IBISBA Workflows, NMR Workflow, UNLOCK, NanoGalaxy, Galaxy Climate, PNDB, IMBforge, COVID-19 PubSeq: Public SARS-CoV-2 Sequence Resource, LBI-RUD, Nick-test-team, Italy-Covid-data-Portal, UX trial team, Integrated and Urban Plant Pathology Laboratory, SARS-CoV-2 Data Hubs, lmjxteam2, virAnnot pipeline, Ay Lab, iPC: individualizedPaediatricCure, Harkany Lab, MOLGENIS, EJPRD WP13 case-studies workflows, Common Workflow Language (CWL) community, Testing, SeBiMER, IAA-CSIC, MAB - ATGC, Probabilistic graphical models, GenX, Snakemake-Workflows, ODA, IPK BIT, CO2MICS Lab, FAME, CHU Limoges - UF9481 Bioinformatique / CNR Herpesvirus, Quadram Institute Bioscience - Bioinformatics, HecatombDevelopment, Institute of Human Genetics, Testing RO Crates, Test Team, Applied Computational Biology at IEG/HMGU, INFRAFRONTIER workflows, OME, TransBioNet, OpenEBench, Bioinformatics and Biostatistics (BIO2 ) Core, VIB Bioinformatics Core, CRC Cohort, ICAN, MustafaVoh, Single Cell Unit, CO-Graph, emo-bon, TestEMBL-EBIOntology, CINECA, Toxicology community, Pitagora-Network, Workflows Australia, Medizinisches Proteom-Center, Medical Bioinformatics, AGRF BIO, EU-Openscreen, X-omics, ELIXIR Belgium, URGI, Size Inc, GA-VirReport Team, The Boucher Lab, Air Quality Prediction, pyiron, CAPSID, Edinburgh Genomics, Defragmentation TS, NBIS, Phytoplankton Analysis, Seq4AMR, Workflow registry test, Read2Map, SKM3, ParslRNA-Seq: an efficient and scalable RNAseq analysis workflow for studies of differentiated gene expression, de.NBI Cloud, Meta-NanoSim, ILVO Plant Health, EMERGEN-BIOINFO, KircherLab, Apis-wings, BCCM_ULC, Dessimoz Lab, TRON gGmbH, GEMS at MLZ, Computational Science at HZDR, Big data in biomedicine, TRE-FX, MISTIC, Guigó lab, Statistical genetics, Delineating Regions-of-interest for Mass Spectrometry Imaging by Multimodally Corroborated Spatial Segmentation, OLCF-WES, Bioinformatics Unit @ CRG, Bioinformatics Innovation Lab, BSC-CES, ELIXIR Proteomics, Black Ochre Data Labs, Zavolan Lab, Metabolomics-Reproducibility, Team Cardio, NGFF Tools, Bioinformatics workflows for life science, Workflows for geographic science, Pacific-deep-sea-sponges-microbiome, CSFG, SNAKE, Katdetectr, INFRAFRONTIER GmbH, PerMedCoE, Euro-BioImaging, EOSC-Life WP3 OC Team, cross RI project, ANSES-Ploufragan, SANBI Pathogen Bioinformatics, Biodata Analysis Group, DeSci Labs, Erasmus MC - Viroscience Bioinformatics, ARA-dev, Mendel Centre for Plant Genomics and Proteomics, Metagenomic tools, WorkflowEng, Polygenic Score Catalog, bpm, scNTImpute, Systems Biotechnology laboratory, Cimorgh IT solutions, MLme: Machine Learning Made Easy, Hurwitz Lab, Dioscuri TDA, Scipion CNB, System Biotechnology laboratory, yPublish - Bioinfo tools, NIH CFDE Playbook Workflow Partnership, MMV-Lab, EMBL-Bioimage Analysis Support, EBP-Nor, Evaluation of Swin Transformer and knowledge transfer for denoising of super-resolution structured illumination microscopy data, Bioinformatics Laboratory for Genomics and Biodiversity (LBGB), multi-analysis dFC, CholGen, RNA group, Plant Genomes Pipelines in Galaxy, Pathogen Genomic Laboratory, Chemical Data Lab, JiangLab, Pangenome database project, HP2NET - Framework for construction of phylogenetic networks on High Performance Computing (HPC) environment, Center for Open Bioimage Analysis, Generalized Open-Source Workflows for Atomistic Molecular Dynamics Simulations of Viral Helicases, Historical DNA genome skimming, QCDIS, Peter Menzel's Team, NHM Clark group, ESRF Workflow System (Ewoks), Kalbe Bioinformatics, Nextflow4Metabolomics, GBCS, CEMCOF, Jackson Laboratory NGS-Ops, Schwartz Lab, BRAIN - Biomedical Research on Adult Intracranial Neoplasms, Cancer Therapeutics and Drug Safety, Deepdefense, Mid-Ohio Regional Planning Commission, MGSSB, Institute for Human Genetics and Genomic Medicine Aachen, FengTaoSMU, EGA, Plant-Food-Research-Open, KrauthammerLab, Geo Workflows, grassland pDT, FunGIALab, CRIM - Computer Research Institute of Montréal, Medvedeva Lab, Metagenlab, FAIR-EASE, Protein-protein and protein-nucleic acid binding site prediction research, Culhane Lab, IDUN - Drug Delivery and Sensing, Edge Computing DAG Task Scheduling Research Group, Stratum corneum nanotexture feature detection using deep learning and spatial analysis: a non-invasive tool for skin barrier assessment, COPO, Taudière group, ErasmusMC Clinical Bioinformatics, interTwin, fluid flow modeling, EnrichDO, WorkflowResearch, Application Security - Test Crypt4GH solutions, RenLabBioinformatics, Yongxin's team, PiFlow, HLee_SeoGroup, UFZ - Image Data Management and Processing Workflows, Korean Bioinformaticians, Into the deep, XChem, CPM, SocialGene, Research Data Management ICE-2, ObjectRecognition, LiDAR, FONDA II C2, Astroparticle Lab, FAIRagro M4.4, Kgerring, QuackenbushLab, Virus sequencing team, SOS, BioImage Informatics and Analysis Workflows, BoostNano, simblockflow, CSSB, Research on Workflow scheduling, Research Data1, CSUbioinformatics, CDPP, Mr., ASD-HRS, data management, FAIR_thesis: Marine acoustic data, CellBinDB, DEEP Lab, University of Amsterdam, SIMEXP, nf-pediatric Team, Kasmanas, Structural Variation Analysis, fuzzyworkflow, CausalCoxMGM Team, Tufts University Center for Cellular Agriculture (TUCCA), Test, CrustyBase, Applied Computational Cancer Research, Click-qPCR, BAID Team, FabianLab, Vector informatics and genomics group, AlmondBreedingLab, Artificial Design for Intelligent Breeding, ELIXIR Biodiversity Community, GROTIA, Biomedical_LLM, WhiteSymmetry, Hämatologie Labor Kiel, pakbaba, Metagenomics Analysis, MTB Bioinformatics Workflows, RTC Bioinformatics, CMG-GUTS, EI Papatheodorou Group, Metagenomics Analysis of Microbiome, ColoPola: A polarimetric imaging dataset for colorectal cancer detection, AI in Zhou Lab, CEPLAS, LTER-Italy, Gevaert Lab, Kendall-Theisen lab pipelines, High Performance Scientific Computing Laboratory (HPSC), Ensembl Metazoa and Plants, BiRD, Q-Dawn, AMRMALDI, Systems and Synthetic Biology, EI Core Bioinformatics Group, Values Shape Community, SolutionMake, GENEX, iPol, zxfenglab, Bioinformatics Unit IIS-FJD, omnibenchmark, BioX Fanatics, Li-Omics Lab, Metro Team, ZiemertLab, Song Lab, PanGIA, Holobionts Workflows, An Ideal Design for RNA-seq investigations into RPL, AMI2B Team, Institute for Hearing Technology and Acoustics (IHTA), Transcriptomics in unexplained recurrent pregnancy loss, E-MOBI / EKONOMIK MOBIL,S.R.L, Workflow Run Crate working group, datafun, BioFAIR, BH2025_Project30, NII Test Team, LIB, Galaxy and Beyond, Biodepot Spatial Proteomics Team, WEHI SODA Hub, Digital Platform, Anesthesia and Neurocritical Care Medicine, Digital Skills in Arts and Humanities, BioimageAIpub, Dalmolin Systems Biology Group, FAIRMD, KG-Microbe, BGIQD, CSB, EMRS, BioImagingUK, Bachelor Thesis Developeming an Algorithm for the Generation of Synthetic Scientific Workflows and Characterization of the generated Workflows, GenotoulBioinfo, mzlab, Liver Polyploidy Research Team, Computational Metabolomics (UoB), UiT-CANS, PathoFact2, Polyploid Spatial Transcriptomics Team, CCBR, FONDA T9 Project Team, Cen Occitanie's Geomateam, Exeter Genetics, HP-Phasing, CPSM, Bayrak Lab, Eco-Flow, oceanbase, Proj Renzo, UFZ Galaxy workflows
Web page: Not specified
Bioinformatics team of the ANSES (French Agency for Food, Environmental and Occupational Health & Safety) of Ploufragan (France)
Space: Independent Teams
Public web page: https://www.anses.fr
Using:
- vadr annotation (model to select)
- vardict variant caller
- coverage depth
Provides summarizing files:
- png image of variant calling with annotations and coverage depths
- tsv file with all information of significant variants only
- vcf file with all information of significant variants only (to allow downstream NextStrain analyses)
Type: Galaxy
Creators: Fabrice Touzain, This study was founded by the French National Research Agency and by Santé publique France as part of the project "EMERGEN". Anses Ploufragan research was also supported by Agglomération de Saint-Brieuc, Département des Côtes d'Armor and Région Bretagne
Submitter: Fabrice Touzain
Using:
- vadr annotation (model to select)
- vardict variant caller
- coverage depth
Provides summarizing files:
- png image of variant calling with annotations and coverage depths
- tsv file with all information of significant variants only
- vcf file with all information of significant variants only (to allow downstream NextStrain analyses)
Type: Galaxy
Creators: Fabrice Touzain, This study was founded by the French National Research Agency and by Santé publique France as part of the project "EMERGEN". Anses Ploufragan research was also supported by Agglomération de Saint-Brieuc, Département des Côtes d'Armor and Région Bretagne
Submitter: Fabrice Touzain
Using:
- vadr annotation (model to select)
- vardict variant caller
- coverage depth
Provides summarizing files:
- png image of variant calling with annotations and coverage depths
- tsv file with all information of significant variants only
- vcf file with all information of significant variants only (to allow downstream NextStrain analyses)
Type: Galaxy
Creators: Fabrice Touzain, This study was founded by the French National Research Agency and by Santé publique France as part of the project "EMERGEN". Anses Ploufragan research was also supported by Agglomération de Saint-Brieuc, Département des Côtes d'Armor and Région Bretagne
Submitter: Fabrice Touzain
Using:
- vadr annotation (model to select)
- vardict variant caller
- coverage depth
Provides summarizing files:
- png image of variant calling with annotations and coverage depths
- tsv file with all information of significant variants only
- vcf file with all information of significant variants only (to allow downstream NextStrain analyses)
Type: Galaxy
Creators: Fabrice Touzain, This study was founded by the French National Research Agency and by Santé publique France as part of the project "EMERGEN". Anses Ploufragan research was also supported by Agglomération de Saint-Brieuc, Département des Côtes d'Armor and Région Bretagne
Submitter: Fabrice Touzain
