Modified workflow to perform Voronoi segmentation, based on https://usegalaxy.eu/published/workflow?id=23030421cd9fcfb2.

Input requirements:

• RA: -- right ascension in degrees as a float number.
• Dec: -- Declination in degrees as a float number
• Radius: -- the radius of the cone to be queried in arcminutes as a float number
• Pixel size: -- the size of the pixel in arcseconds
• Band: -- the band (channel) of the image: g, r, z, i

The present workflow allows to perform 2D spots/blobs detection on specific channel and stack of an image. Images will be directly fetched from IDR by including the IDs. A pre-processing steps with histogram normalization is performed before the blobs detection.

Associated Tutorial

This workflows is part of the tutorial Quantification of single-molecule RNA fluorescence in situ hybridization (smFISH) in yeast cell lines, ...

Generic workflow to perform Voronoi segmentation. Input requirements:

• Image: -- Preferably lighter objects on a darker background for the mask to work well. -- Format: .tiff, stored in planar RGB format, not interleaved (http://avitevet.com/uncategorized/when-to-use-it-interleaved-vs-planar-image-data-storage/).
• Seeds: -- White seeds on a black background -- Format: .tiff

Associated Tutorial

This workflows is part of the tutorial [Voronoi ...

[![Cite with ...