Proteogenomics 1: Database Creation
10.0

Workflow Type: Galaxy

Associated Tutorial

This workflows is part of the tutorial Proteogenomics 1: Database Creation, available in the GTN

Features

Thanks to...

Tutorial Author(s): Subina Mehta, Timothy J. Griffin, Pratik Jagtap, Ray Sajulga, James Johnson, Praveen Kumar

gtn star logo followed by the word workflows

Inputs

ID Name Description Type
FASTQ_ProB_22LIST.fastqsanger FASTQ_ProB_22LIST.fastqsanger n/a
  • File
Reference Annotation Reference Annotation n/a
  • File
Reference Genome Reference Genome n/a
  • string
Reference Genome Annotation for CustomProDB Reference Genome Annotation for CustomProDB n/a
  • string
Trimmed_ref_5000_uniprot_cRAP.fasta Trimmed_ref_5000_uniprot_cRAP.fasta n/a
  • File

Steps

ID Name Description
5 Filter Tabular toolshed.g2.bx.psu.edu/repos/iuc/filter_tabular/filter_tabular/3.3.1
6 Replace Text toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_column/9.5+galaxy0
7 HISAT2 toolshed.g2.bx.psu.edu/repos/iuc/hisat2/hisat2/2.2.1+galaxy1
8 FreeBayes toolshed.g2.bx.psu.edu/repos/devteam/freebayes/freebayes/1.3.9+galaxy0
9 StringTie toolshed.g2.bx.psu.edu/repos/iuc/stringtie/stringtie/2.2.3+galaxy0
10 CustomProDB toolshed.g2.bx.psu.edu/repos/galaxyp/custom_pro_db/custom_pro_db/1.22.0
11 GffCompare toolshed.g2.bx.psu.edu/repos/iuc/gffcompare/gffcompare/0.12.6+galaxy0
12 FASTA Merge Files and Filter Unique Sequences toolshed.g2.bx.psu.edu/repos/galaxyp/fasta_merge_files_and_filter_unique_sequences/fasta_merge_files_and_filter_unique_sequences/1.2.0
13 SQLite to tabular toolshed.g2.bx.psu.edu/repos/iuc/sqlite_to_tabular/sqlite_to_tabular/3.2.1
14 SQLite to tabular toolshed.g2.bx.psu.edu/repos/iuc/sqlite_to_tabular/sqlite_to_tabular/3.2.1
15 FASTA-to-Tabular toolshed.g2.bx.psu.edu/repos/devteam/fasta_to_tabular/fasta2tab/1.1.1
16 Convert gffCompare annotated GTF to BED toolshed.g2.bx.psu.edu/repos/galaxyp/gffcompare_to_bed/gffcompare_to_bed/0.2.1
17 FASTA-to-Tabular toolshed.g2.bx.psu.edu/repos/devteam/fasta_to_tabular/fasta2tab/1.1.1
18 Column Regex Find And Replace toolshed.g2.bx.psu.edu/repos/galaxyp/regex_find_replace/regexColumn1/1.0.3
19 Column Regex Find And Replace toolshed.g2.bx.psu.edu/repos/galaxyp/regex_find_replace/regexColumn1/1.0.3
20 Filter Tabular toolshed.g2.bx.psu.edu/repos/iuc/filter_tabular/filter_tabular/3.3.1
21 Translate BED transcripts toolshed.g2.bx.psu.edu/repos/galaxyp/translate_bed/translate_bed/0.1.0
22 Column Regex Find And Replace toolshed.g2.bx.psu.edu/repos/galaxyp/regex_find_replace/regexColumn1/1.0.3
23 Query Tabular toolshed.g2.bx.psu.edu/repos/iuc/query_tabular/query_tabular/3.3.2
24 Concatenate multiple datasets toolshed.g2.bx.psu.edu/repos/artbio/concatenate_multiple_datasets/cat_multi_datasets/1.4.3
25 bed to protein map toolshed.g2.bx.psu.edu/repos/galaxyp/bed_to_protein_map/bed_to_protein_map/0.2.0
26 Tabular-to-FASTA toolshed.g2.bx.psu.edu/repos/devteam/tabular_to_fasta/tab2fasta/1.1.1
27 Concatenate multiple datasets toolshed.g2.bx.psu.edu/repos/artbio/concatenate_multiple_datasets/cat_multi_datasets/1.4.3
28 FASTA Merge Files and Filter Unique Sequences toolshed.g2.bx.psu.edu/repos/galaxyp/fasta_merge_files_and_filter_unique_sequences/fasta_merge_files_and_filter_unique_sequences/1.2.0
29 Query Tabular toolshed.g2.bx.psu.edu/repos/iuc/query_tabular/query_tabular/3.3.2

Outputs

ID Name Description Type
Reference Annotation fixed.gtf Reference Annotation fixed.gtf n/a
  • File
HISAT_Output.BAM HISAT_Output.BAM n/a
  • File
Stringtie_output.gtf Stringtie_output.gtf n/a
  • File
transcripts_annotated transcripts_annotated n/a
  • File
Merged and Filtered FASTA from CustomProDB Merged and Filtered FASTA from CustomProDB n/a
  • File
genomic_mapping_sqlite genomic_mapping_sqlite n/a
  • File
variant_annotation_sqlite variant_annotation_sqlite n/a
  • File
SAV_INDEL SAV_INDEL n/a
  • File
variant_annotation variant_annotation n/a
  • File
Translate cDNA_minus_CDS Translate cDNA_minus_CDS n/a
  • File
Variant_annotation_sqlitedb Variant_annotation_sqlitedb n/a
  • File
CustomProDB Merged Fasta CustomProDB Merged Fasta n/a
  • File
Genomic_Protein_map Genomic_Protein_map n/a
  • File
Uniprot_cRAP_SAV_indel_translatedbed Uniprot_cRAP_SAV_indel_translatedbed n/a
  • File
genomic_mapping_sqlitedb genomic_mapping_sqlitedb n/a
  • File

Version History

10.0 (earliest) Created 2nd Jun 2025 at 10:51 by GTN Bot

Added/updated 4 files


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Created: 2nd Jun 2025 at 10:51

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