WF3_VERIFICATION_WORKFLOW
1.0

Workflow Type: Galaxy

WF3- Peptide verification/validaiton workflow

Associated Tutorial

This workflows is part of the tutorial Clinical Metaproteomics 3: Verification, available in the GTN

Thanks to...

Workflow Author(s): Subina Mehta

Tutorial Author(s): Subina Mehta, Katherine Do, Dechen Bhuming

Tutorial Contributor(s): Pratik Jagtap, Timothy J. Griffin, Bérénice Batut, Helena Rasche, Saskia Hiltemann, Björn Grüning, Subina Mehta

gtn star logo followed by the word workflows

Inputs

ID Name Description Type
Distinct Peptides for PepQuery Distinct Peptides for PepQuery n/a
  • File
Input MGFs Dataset Collection Input MGFs Dataset Collection n/a
  • File[]
MaxQuant-peptide-report MaxQuant-peptide-report n/a
  • File
SGPS_peptide-report SGPS_peptide-report n/a
  • File

Steps

ID Name Description
0 Human UniProt+Isoforms FASTA toolshed.g2.bx.psu.edu/repos/galaxyp/dbbuilder/dbbuilder/0.3.4
1 cRAP toolshed.g2.bx.psu.edu/repos/galaxyp/dbbuilder/dbbuilder/0.3.4
6 Human UniProt+Isoforms+cRAP FASTA toolshed.g2.bx.psu.edu/repos/galaxyp/fasta_merge_files_and_filter_unique_sequences/fasta_merge_files_and_filter_unique_sequences/1.2.0
7 Cut Cut1
8 Cut Cut1
9 PepQuery2 toolshed.g2.bx.psu.edu/repos/galaxyp/pepquery2/pepquery2/2.0.2+galaxy0
10 Remove beginning Remove beginning1
11 Remove beginning Remove beginning1
12 Collapse Collection toolshed.g2.bx.psu.edu/repos/nml/collapse_collections/collapse_dataset/5.1.0
13 Concatenate datasets toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cat/0.1.1
14 Filter Filter1
15 Remove beginning Remove beginning1
16 Cut Cut1
17 Peptide and Protein from Peptide reports toolshed.g2.bx.psu.edu/repos/iuc/query_tabular/query_tabular/3.3.0
18 Remove beginning Remove beginning1
19 Group Grouping1
20 Uniprot-ID from verified Peptides toolshed.g2.bx.psu.edu/repos/iuc/query_tabular/query_tabular/3.3.0
21 UniProt toolshed.g2.bx.psu.edu/repos/galaxyp/uniprotxml_downloader/uniprotxml_downloader/2.4.0
22 Quantitation Database for MaxQuant toolshed.g2.bx.psu.edu/repos/galaxyp/fasta_merge_files_and_filter_unique_sequences/fasta_merge_files_and_filter_unique_sequences/1.2.0

Outputs

ID Name Description Type
Human UniProt+Isoforms FASTA Human UniProt+Isoforms FASTA n/a
  • File
cRAP cRAP n/a
  • File
Human UniProt+Isoforms+cRAP FASTA Human UniProt+Isoforms+cRAP FASTA n/a
  • File
Peptide and Protein from Peptide reports Peptide and Protein from Peptide reports n/a
  • File
Uniprot-ID from verified Peptides Uniprot-ID from verified Peptides n/a
  • File
Quantitation Database for MaxQuant Quantitation Database for MaxQuant n/a
  • File

Version History

1.0 (earliest) Created 2nd Jun 2025 at 10:52 by GTN Bot

Added/updated 4 files


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Created: 2nd Jun 2025 at 10:52

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