Combining datasets after pre-processing
1.0

Workflow Type: Galaxy

Updated March 2024

Associated Tutorial

This workflows is part of the tutorial Combining single cell datasets after pre-processing, available in the GTN

Thanks to...

Workflow Author(s): Julia Jakiela, Wendi Bacon

Tutorial Author(s): Wendi Bacon

Tutorial Contributor(s): Helena Rasche, Jonathan Manning, Julia Jakiela, Pavankumar Videm, Saskia Hiltemann, Björn Grüning, Marisa Loach, Wendi Bacon, Bérénice Batut, Mehmet Tekman, Pablo Moreno

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Inputs

ID Name Description Type
N701 N701 Dataset to be combined
  • File
N702 N702 Dataset to be combined
  • File
N703 N703 Dataset to be combined
  • File
N704 N704 Dataset to be combined
  • File
N705 N705 Dataset to be combined
  • File
N706 N706 Dataset to be combined
  • File
N707 N707 Dataset to be combined
  • File

Steps

ID Name Description
7 Concatenating Datasets toolshed.g2.bx.psu.edu/repos/iuc/anndata_manipulate/anndata_manipulate/0.10.3+galaxy0
8 Inspect AnnData toolshed.g2.bx.psu.edu/repos/iuc/anndata_inspect/anndata_inspect/0.10.3+galaxy0
9 Inspect AnnData toolshed.g2.bx.psu.edu/repos/iuc/anndata_inspect/anndata_inspect/0.10.3+galaxy0
10 Inspect AnnData toolshed.g2.bx.psu.edu/repos/iuc/anndata_inspect/anndata_inspect/0.10.3+galaxy0
11 Replace Text toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_column/9.3+galaxy0
12 Replace Text toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_column/9.3+galaxy0
13 Cut Cut1
14 Cut Cut1
15 Paste Paste1
16 Adding in cell metadata toolshed.g2.bx.psu.edu/repos/iuc/anndata_manipulate/anndata_manipulate/0.10.3+galaxy0
17 Batching Cell Metadata toolshed.g2.bx.psu.edu/repos/iuc/anndata_manipulate/anndata_manipulate/0.10.3+galaxy0
18 Calculating mitochondrial toolshed.g2.bx.psu.edu/repos/ebi-gxa/anndata_ops/anndata_ops/1.9.3+galaxy0

Outputs

ID Name Description Type
Combined Object Combined Object n/a
  • File
Sex metadata Sex metadata n/a
  • File
Genotype metadata Genotype metadata n/a
  • File
Cell metadata Cell metadata n/a
  • File
AnnData with Cell Metadata AnnData with Cell Metadata n/a
  • File
Batched Object Batched Object n/a
  • File
Annotated AnnData Annotated AnnData n/a
  • File

Version History

1.0 (earliest) Created 2nd Jun 2025 at 10:57 by GTN Bot

Added/updated 4 files


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Created: 2nd Jun 2025 at 10:57

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