Workflow Constructed From History 'STACKS RAD: Population Genomics With Reference Genome'
3.0

Workflow Type: Galaxy

RAD-Seq Reference-based data analysis

Associated Tutorial

This workflows is part of the tutorial RAD-Seq Reference-based data analysis, available in the GTN

Thanks to...

Tutorial Author(s): Yvan Le Bras

Tutorial Contributor(s): Verena Moosmann, Helena Rasche, Saskia Hiltemann, Nicola Soranzo, Yvan Le Bras, Bérénice Batut, Björn Grüning, William Durand, Niall Beard, Wolfgang Maier

gtn star logo followed by the word workflows

Inputs

ID Name Description Type
SRR034310.fastq.gz EBI SRA: SRR034310 File: ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR034/SRR034310/SRR034310.fastq.gz n/a
  • File
Barcode_SRR034310.txt https://zenodo.org/record/1134547/files/Barcode_SRR034310.txt n/a
  • File
Details_Barcode_Population_SRR034310.txt https://zenodo.org/record/1134547/files/Details_Barcode_Population_SRR034310.txt n/a
  • File
Reference_genome_11_chromosomes.fasta https://zenodo.org/record/1134547/files/Reference_genome_11_chromosomes.fasta n/a
  • File

Steps

ID Name Description
10 FastQC toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.71
11 Map with BWA toolshed.g2.bx.psu.edu/repos/devteam/bwa/bwa/0.7.17.4
12 Select Grep1
13 Select Grep1
14 Replace Text toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.1
15 Replace Text toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.1
16 Replace Text toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.1
17 MultiQC toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.6
18 Replace Text toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.1
19 Replace Text toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.1
20 Add column addValue
21 Concatenate datasets cat1
22 Cut Cut1
23 Convert Convert characters1
24 Regex Replace toolshed.g2.bx.psu.edu/repos/kellrott/regex_replace/regex_replace/1.0.0
25 Stacks: reference map toolshed.g2.bx.psu.edu/repos/iuc/stacks_refmap/stacks_refmap/1.46.0
26 Stacks: populations toolshed.g2.bx.psu.edu/repos/iuc/stacks_populations/stacks_populations/1.46.0
27 Summary Statistics Summary_Statistics1
28 Sort toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sort_header_tool/1.1.1
29 Count Count1
30 Filter Filter1
4 Stacks: process radtags toolshed.g2.bx.psu.edu/repos/iuc/stacks_procrad/stacks_procrad/1.46.0
5 Stacks: process radtags toolshed.g2.bx.psu.edu/repos/iuc/stacks_procrad/stacks_procrad/1.46.0
6 Stacks: process radtags toolshed.g2.bx.psu.edu/repos/iuc/stacks_procrad/stacks_procrad/1.46.0
7 Replace Text toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_replace_in_line/1.1.1
8 Select Grep1
9 Select Grep1

Version History

3.0 (earliest) Created 2nd Jun 2025 at 11:03 by GTN Bot

Added/updated 4 files


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Created: 2nd Jun 2025 at 11:03

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