Workflows
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FREEPII (Feature Representation Enhancement End-to-end Protein Interaction Inference) is an end-to-end learning method encompassing autonomous feature extraction and feature representation enhancement for PPIs and protein complexes inference.
Portable genotype-free demultiplexing benchmarkign pipeline.
A portable pipeline for benchmarking genotype-free single-cell demultiplexing methods on simulated data.
The pipeline is designed to be generelisable to different datasets with arbitrary numbers of simulated mulitplexed samples. All software as part of pipeline is run through Apptainer containers to ensure reproducibility and ease of use. The pipeline default configuration is to be run on a cluster with a SLURM scheduler, but can be ...
Type: Nextflow
Creators: Michael P Lynch, Leverages scripts developed by Weber et al (2021) DOI: https://doi.org/10.1093/gigascience/giab062
Submitter: Michael Lynch
Classification and visualization of SSU, LSU sequences.
Associated Tutorial
This workflows is part of the tutorial MGnify v5.0 Amplicon Pipeline, available in the GTN
Features
- Includes a Galaxy Workflow Report
- Uses ...
demux_doublet_sim
Repository for Nextflow pipeline used in demuxSNP demultipelxing paper
Overall workflow
- Simulate doublets
- Add per-sample suffix to barcodes in BAM
- Merge per-sample BAMs
- Generate lookup of barcodes to rename to reach a set % doublets
- Rename barcodes in BAM as per lookup
- Benchmark methods
- Experiments 1: Vary doublet rate
- Experiment 2: Vary SNP subsetting
Inputs
Most inputs are specified in nextflow.config: container__souporcell: path to souporcell apptainer ...
Type: Nextflow
Creators: Michael Lynch, Leverages scripts developed by Weber et al (2021) DOI: https://doi.org/10.1093/gigascience/giab062
Submitter: Michael Lynch
PaSTa is a nextflow-based end-to-end image analysis pipeline for decoding image-based spatial transcriptomics data. It performs imaging cycle registration, cell segmentation and transcripts peak decoding. It is currently supports analysis of three types of ST technology:
- in-situ sequencing-like encoding
- MERFISH-like encoding
- RNAScope-like labelling
Prerequisites:
- Nextflow. Installation guide: https://www.nextflow.io/docs/latest/getstarted.html
- Docker or Singularity. Installation guide: ...
YOLO based tail analysis workflow for image segmentation and detection. The workflow is based on https://git.embl.org/grp-cba/tail-analysis/-/blob/main/analysis_workflow.md
Associated Tutorial
This workflows is part of the tutorial Training Custom YOLO Models for Object Detection and Segmentation in Bioimages, available in the GTN
...
From Obis data to Biodiversity indicators
Associated Tutorial
This workflows is part of the tutorial Obis marine indicators, available in the GTN
Features
- Includes Galaxy Workflow Tests
Thanks to...
Workflow Author(s): Marie Jossé
Tutorial Author(s): ...
Tests